Mouse Oocyte Development: Methods and Protocols (Methods in Molecular Biology)

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.





Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.

1. Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse

Stephany El-Hayek, Qin Yang, and Hugh J. Clarke

2. In vitro Culture of Mouse Oocytes for Meiotic Maturation

Ewa Borsuk and Jacek Z. Kubiak

3. In vitro Maturation and Culture of Human Oocytes

Jennifer R Gruhn, Stine Gry Kristensen, Claus Yding Andersen, and Eva R Hoffmann

4. Pronuclear Transfer in Human Oocytes

Louise Hyslop

5. In vitro Fertilization of Mouse Oocyte

Agathe Chaigne

6. Profiling Maternal mRNA Translation during Oocyte Development

Joao P. Sousa Martins and Marco Conti

7. Transcriptome Profiling of Single Mouse Oocytes

Maud Borensztein , Laurène Syx , Nicolas Servant, and Edith Heard

8. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation

Cecilia S. Blengini and Karen Schindler

9. Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation

Jessica Greaney, Zhe Wei, and Hayden Homer

10. Triple-colour Live Imaging of Mouse Oocytes

Aurélien Courtois, Petr Solc, and Tomoya S. Kitajima

11. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes

Elvira Nikalayevich, Nora Bouftas, and Katja Wassmann

12. Manipulating Cohesin Levels in Live Mouse Oocytes

Anna Szydlowska, Sabrina Ladstätter, and Kikuë Tachibana

13. Optogenetic Manipulation of Mouse Oocytes

Takashi Akera, David M. Chenoweth, and Michael A. Lampson

14. Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin

Greg FitzHarris

15. Photoactivation of Actin in Mouse Oocyte

Maria Almonacid

16. Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes

Isma Bennabi and Marion Manil-Ségalen

17. Micropipette Aspiration of Oocytes to Assess Cortical Tension

Janice P. Evans and Douglas N. Robinson