Handbook of Nucleic Acid Purification by Dongyou Liu (English) Hardcover Book

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Covers nucleic acid purification strategies for bacteria, viruses, fungi, parasites, mammals, and plants. This book emphasizes sample preparation methods for direct molecular applications. It provides a review of historical background and purification principles.

FORMATHardcover LANGUAGEEnglish CONDITIONBrand New Publisher Description

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods.It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

Author Biography

Human Genetic Signatures, North Ryde, NSW, Australia

Table of Contents

Purification of Nucleic Acids from Bacteria. Purification of Nucleic Acids from Viruses. Purification of Nucleic Acids from Fungi. Purification of Nucleic Acids from Parasites. Purification of Nucleic Acids from Mammals. Purification of Nucleic Acids from Plants. Purification of Nucleic Acids from Miscellaneous Sources. Index.

Review

"I was very impressed with the amount of technical detail in the book, having comprehensive descriptions of in-house and commercial methods, together with consideration of the theoretical basis of methods. There is so much more to this book than merely being a laboratory manual. The chapters are very well written, reference lists are comprehensive, and figures and diagrams are good." – Vince Mulholland, SASA, in Microbiology Today, May 2009

Details ISBN1420070967 Author Dongyou Liu Short Title HANDBK OF NUCLEIC ACID PURIFIC Pages 584 Language English ISBN-10 1420070967 ISBN-13 9781420070965 Media Book Format Hardcover DEWEY 572.33 Year 2009 Imprint CRC Press Inc Country of Publication United States Edited by Dongyou Liu Edition 1st Place of Publication Bosa Roca Affiliation RCPA Biosecurity QAP, New South Wales, Australia DOI 10.1604/9781420070965 AU Release Date 2009-01-14 NZ Release Date 2009-01-14 US Release Date 2009-01-14 UK Release Date 2009-01-14 Publisher Taylor & Francis Inc Publication Date 2009-01-14 Alternative 9781032297545 Illustrations 53 Illustrations, black and white Audience Professional & Vocational We've got this

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