The Nile on eBay FREE SHIPPING UK WIDE Handbook of ELISPOT by Alexander E. Kalyuzhny
Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.
FORMATHardcover LANGUAGEEnglish CONDITIONBrand New Publisher Description
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.
Notes
Extends and expands upon the hands-on methodologies of the popular first editionFeatures expert techniques ready for the laboratoryIncludes key tips and implementation advice to ensure successful results
Back Cover
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of Handbook of ELISPOT: Methods and Protocols , only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.
Table of Contents
Unique Strengths of ELISPOT for T Cell Diagnostics.- The Impact of Harmonization on ELISPOT Assay Performance.- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells.- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes.- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay.- Dual and Triple Color Fluorospot.- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes.- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells.- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion.- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell.- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity.- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay.- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles.- Statistical Analysis of ELISPOT Assays.- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories.- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT.- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis.- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®.- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays.
Review
From the reviews of the second edition:"In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple." (Hayley Evans, Immunology News, November, 2012)
Review Quote
From the reviews of the second edition:In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple. (Hayley Evans, Immunology News, November, 2012)
Feature
Extends and expands upon the hands-on methodologies of the popular first edition Features expert techniques ready for the laboratory Includes key tips and implementation advice to ensure successful results
Details ISBN1617793248 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2011 Edition 2nd ISBN-10 1617793248 ISBN-13 9781617793240 Format Hardcover Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Alexander E. Kalyuzhny DEWEY 616.079 Short Title HANDBK OF ELISPOT 2012/E 2/E Language English Media Book Series Number 792 Subtitle Methods and Protocols Replaces 9781588294692 Publication Date 2011-09-29 Pages 261 DOI 10.1007/978-1-61779-325-7 AU Release Date 2011-09-29 NZ Release Date 2011-09-29 US Release Date 2011-09-29 UK Release Date 2011-09-29 Author Alexander E. Kalyuzhny Edition Description 2nd ed. 2012 Alternative 9781493961115 Audience Professional & Vocational Illustrations 13 Illustrations, color; 46 Illustrations, black and white; X, 261 p. 59 illus., 13 illus. in color. We've got this
At The Nile, if you're looking for it, we've got it.With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love!
30 DAY RETURN POLICY
No questions asked, 30 day returns!
FREE DELIVERY
No matter where you are in the UK, delivery is free.
SECURE PAYMENT
Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137905086;
Your request is processing... Please Wait